|Minimum Order Quantity||1 Piece|
|Temperature Range||0- 25 deg C|
|Suitable For||RS Analysis|
We are offering Chromasol HPLC Column. These can be used in any brand of HPLC instruments with high resolution, durability and accurate peak shape. Please let us know the list of columns you are currently procuring for routine analysis, we would like you to give us an opportunity to provide an equivalent product having the same chemistry with excellent quality and performance.
High performance liquid chromatography (HPLC) is a type of liquid chromatography that uses a liquid moblie phase. The same basic principals from gas chromatography are applied to liquid chromatography. There are three basic types of liquid chromatographic columns: liquid-liquid, liquid-solid, and ion-exchange. Liquid-liquid chromatographic columns have the liquid stationary phase bonded or absorbed to the surface of the column, or packed material. liquid-liquid chromatographic columns are not as popular because they have limited stability and they are inconvenient. Partitioning occurs between the two different liquids of the mobile and stationary phases. In liquid-solid chromatographic columns the stationary phase is a solid and the analyte absorbs onto the stationary phase which separates the components of the mixture. In ion-exchange chromatographic columns the stationary phase is an ion-exchange resin and partitioning occurs with ion exchanges that occur between the analyte and stationary phase.
Usually HPLC has a guard column ahead of the analytical column to protect and extend the life of the analytical column. The guard column removes particulate matter, contaminants, and molecules that bind irreversibly to the column. The guard column has a stationary phase similar to the analytical column.
The most common HPLC columns are made from stainless steel, but they can be also made out of thick glass, polymers such as polyetherethelketone, a combination of stainless steel and glass, or a combination of stainless steel and polymers. Typical HPLC analytical columns are between 3 and 25 cm long and have a diameter of 1 to 5 mm. The columns are usually straight unlike GC columns. Particles that pack the columns have a typical diameter between 3 to 5 µm. Liquid chromatographic columns will increase in efficiency when the diameter of the packed particles inside the column decreases.Packing Material
HPLC columns are usually packed with pellicular, or porous particles. Pellicular particles are made from polymer, or glass beads. Pellicular particles are surrounded by a thin uniform layer of silica, polystyrene-divinyl-benzene synthetic resin, alumina, or other type of ion-exchange resin. The diameter of the pellicular beads is between 30 and 40 µm. Porous particles are more commonly used and have diameters between 3 to 10 µm. Porous particles are made up silica, polystyrene-divinyl-benzene synthetic resin, alumina, or other type of ion-exchange resin. Silica is the most common type of porous particle packing material.
Partition HPLC uses liquid bonded phase columns, where the liquid stationary phase is chemically bonded to the packing material. The packing material is usually hydrolyzed silica which reacts with the bond-phase coating. Common bond phase coatings are siloxanes.